This article [attached] is a good follow-up to yesterday’ article on the human interactome in which a “subcellular map of the human proteome” was mentioned. Resolving the spatial distribution –– of the human proteome at a subcellular level –– can greatly increase our understanding of human biology and disease.
Authors [in attached article] present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enables the definition of the proteomes of 13 major organelles.
Exploration of the proteomes revealed single-cell variations in abundance, and/or spatial distribution and localization, of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein-interaction networks and provides an important resource to dissect the highly complex architecture of the human individual cell.